Bacterial survival in evaporating deposited droplets on a teflon-coated surface
Identifieur interne : 004341 ( Main/Exploration ); précédent : 004340; suivant : 004342Bacterial survival in evaporating deposited droplets on a teflon-coated surface
Auteurs : Xiaojian Xie [République populaire de Chine] ; Yuguo Li [République populaire de Chine, Hong Kong] ; Tong Zhang [République populaire de Chine] ; Herbert H. P. Fang [République populaire de Chine]Source :
- Applied Microbiology and Biotechnology [ 0175-7598 ] ; 2006-12-01.
English descriptors
Abstract
Abstract: Understanding of bacterial survival in aerosols is crucial for controlling infection transmission via airborne aerosols and/or large droplets routes. The cell viability changes of four bacteria species (Escherichia coli K12 JM109; Acinetobacter sp. 5A5; Pseudomonas oleovorans X5; and Staphylococcus aureus X8), three Gram-negative and one Gram-positive, in a large evaporating droplet of size 1,800 μm in diameter on teflon-coated slides were measured using the LIVE/DEAD BacLight solution and a microscope. Droplets of three levels of salinity (0, 0.9, and 36% w/v) were tested. All four species survived well during the droplet evaporation process, but died mostly at the time when droplets were dried out at 40–45 min. The final bacteria survival rate after droplets were completely dried was dependent on bacteria species and the salinity of the suspension solution. Droplet evaporation over the first 35–40 min had no adverse effect on bacterial survival for the droplets tested. The lethal effect of desiccation was found to be the most important death mechanism.
Url:
DOI: 10.1007/s00253-006-0492-5
Affiliations:
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Fang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Environmental Biotechnology Laboratory, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Applied Microbiology and Biotechnology</title><title level="j" type="abbrev">Appl Microbiol Biotechnol</title><idno type="ISSN">0175-7598</idno><idno type="eISSN">1432-0614</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2006-12-01">2006-12-01</date><biblScope unit="volume">73</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="703">703</biblScope><biblScope unit="page" to="712">712</biblScope></imprint><idno type="ISSN">0175-7598</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-7598</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial survival</term><term>Desiccation effect</term><term>Droplet</term><term>Evaporation effect</term><term>Salinity effect</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Understanding of bacterial survival in aerosols is crucial for controlling infection transmission via airborne aerosols and/or large droplets routes. The cell viability changes of four bacteria species (Escherichia coli K12 JM109; Acinetobacter sp. 5A5; Pseudomonas oleovorans X5; and Staphylococcus aureus X8), three Gram-negative and one Gram-positive, in a large evaporating droplet of size 1,800 μm in diameter on teflon-coated slides were measured using the LIVE/DEAD BacLight solution and a microscope. Droplets of three levels of salinity (0, 0.9, and 36% w/v) were tested. All four species survived well during the droplet evaporation process, but died mostly at the time when droplets were dried out at 40–45 min. The final bacteria survival rate after droplets were completely dried was dependent on bacteria species and the salinity of the suspension solution. Droplet evaporation over the first 35–40 min had no adverse effect on bacterial survival for the droplets tested. 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